An experiment presenting the effect of temperature changes on enzyme activity

Enzyme activity increases as temperature increases, and in turn increases the rate of the reaction.

An experiment presenting the effect of temperature changes on enzyme activity

Term Papers Tagged With: Enzyme 8 pages, words Qualitative Observations: C have an effect on the activity of the enzyme catalase found in beef liver in the breakdown of hydrogen peroxide?

Hypothesis If liver is placed in different temperatures of 0? C and hydrogen peroxide is added to each piece of liver, then the liver placed in 37? C will have the largest reaction height.

The liver in 90? C will have the smallest reaction height, followed by the liver in 0? C, and then 21?

An experiment presenting the effect of temperature changes on enzyme activity

Background Information Pearson Baccalaureate: Most enzymes are proteins that have Introduction Molecules called enzymes help catalyze reactions. A substrate is the molecule Enzymes are proteins; therefore the liver has a particularly high concentration of catalase.

When hydrogen peroxide H2O2 is added to liver, catalase catalyzes a reaction in which the hydrogen peroxide is broken down into oxygen gas O2 and liquid water H2O.

Hydrogen peroxide is a toxic chemical that is produced as a byproduct of many normal cellular reactions, so it is crucial that catalase in the liver breaks the hydrogen peroxide down into the two harmless substances of oxygen gas and liquid water.

The hydrogen peroxide must be quickly degraded or converted, and catalase accomplishes this task because one molecule of catalase can deal with six million molecules of hydrogen peroxide in one minute.

The prediction in the hypothesis is based on the concept of denaturation. That limit is based on the temperature at which the enzyme as a protein begins to lose its three-dimensional shape due to intramolecular bonds being stressed and broken.

Due to denaturation, the liver placed in ? C will have the smallest reaction height because at this temperature catalase will begin to denature.

C will have small reaction heights, but the heights will increase as temperature increases The liver placed in 37? Although this lab is using beef liver instead of a human liver, the optimum temperature for beef liver should be similar to that of humans.

An experiment presenting the effect of temperature changes on enzyme activity

The predictive graph on the previous page represents how as the temperature of beef liver originally increases, the height of the reaction increases as well due to an increase in molecular collisions.

At a certain temperature, the catalase will reach its optimum temperature and have the greatest height of reaction.

Enzymes such as catalase are protein molecules. They are used to speed up specific reactions in the cells. I will do this experiment at room temperature so the enzymes get enough kinetic energy to collide. However, as the temperature continues to increase the enzyme will begin to lose its shape and denature, so the height of the reaction will decrease.

This lab will be using beef liver, which contains the specific enzyme catalase, and by placing the liver into different temperatures it will be assessed how catalase performs under certain conditions.

After the liver has been placed in different temperatures, hydrogen peroxide will be added to each piece of liver, and by measuring the height of the chemical reaction it will be determined which temperatures catalase performs the best in.

Reactions with a high height will represent catalase quickly and efficiently breaking down the hydrogen peroxide, and reactions with a low height will represent catalase slowly and inefficiently breaking down the hydrogen peroxide.

This lab will serve as a model for the role of enzymes in the human body, and will outline the importance of enzymes for the human body. Investigation Variables Independent Variables The independent variable is the temperature of the liver, and it will be measured in?

The different temperatures used will be 0? Dependent Variables The dependent variable is the height of the reaction, and the bubbles of gas produced will be measured in millimetres mm.

Control variables and their treatments Variable Effect Control Size of liver Could increase or decrease the height of the reaction. A larger piece of liver means more catalase, which could break down the H2O2 at a more efficient rate than smaller pieces of liver.Jul 20,  · Chitosanase activity from T.

koningiiI was also strongly affected by pH and enzyme activity was enhanced at pH values above presenting maximal values around pH and temperature in the range of 40–50°C.

Investigating effect of temperature on the activity of lipase | Nuffield Foundation

DISCUSSION The effect of temperature on the activity of the enzyme, trypsin 1) Set up a water bath at 15°c 2) Pipette 3cm^3 of casein suspension into one test tube and 3cm^3 of trypsin solution into another tube 3) Stand both tubes in the water bath and leave them for several minutes to reach the temperature of the water bath 4) Meanwhile, set up a .

TEMPERATURE EFFECT AT DIFFERENT HYDROGEN ION CONCENTRATIONS. IV. A THEORY OF THE CATALASE REACTION. The study of the influence of changes in hydrogen sum up our experience along this line by presenting a slightly schematized curve of catalase activity at different pH values and temperatures.

Demonstrate the activity of an enzyme in living tissue, observe the effects of changes in temperature and pH on the activity of an enzyme, perform analyses for the presence of an enzyme in tissues, and analyzing relationships between environmental conditions and enzyme activity.

The Effects of Temperature on Enzyme Activity and Biology | Sciencing

Background: Cells produce. Measure the effects of changes in temperature, pH, and enzyme concentration on reaction rates of an enzyme catalyzed reaction in a controlled experiment. Explain how environmental factors affect the rate of enzyme-catalyzed reactions. Investigate the effect of temperature on amylase activity 1.

Investigate the effect of temperature on amylase activityIntroduction Amylase is an enzyme that catalyses the breakdown of starch into sugars.

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